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验证数据展示

  • WB analysis using 82857-2-RR (same clone as 82857-2-PBS)

    WB analysis using 82857-2-RR (same clone as 82857-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82857-2-RR (FBXO44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82857-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82857-2-RR (same clone as 82857-2-PBS)

    IHC staining of mouse brain using 82857-2-RR (same clone as 82857-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82857-2-RR (FBXO44 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82857-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82857-2-RR (same clone as 82857-2-PBS)

    IHC staining of mouse brain using 82857-2-RR (same clone as 82857-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82857-2-RR (FBXO44 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82857-2-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 82857-2-RR (same clone as 82857-2-PBS)

    IF Staining of A431 using 82857-2-RR (same clone as 82857-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FBXO44 antibody (82857-2-RR, Clone: 230229G7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82857-2-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82857-2-RR (same clone as 82857-2-PBS)

    FC experiment of A431 using 82857-2-RR (same clone as 82857-2-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug FBXO44 Recombinant antibody (82857-2-RR, Clone:230229G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82857-2-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 82857-2-RR (same clone as 82857-2-PBS)

    IF Staining of U2OS using 82857-2-RR (same clone as 82857-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXO44 antibody (82857-2-RR, Clone: 230229G7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82857-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82857-2-RR

    Affinity and Kinetic Characterization of 82857-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82857-2-RR against Human FBXO44 were performed. The affinity constant is 0.134 nM.

FBXO44 Recombinant monoclonal antibody, PBS Only

FBXO44 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82857-2-PBS

产品说明书

CloneNo. 230229G7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

230229G7, F box only protein 44, F box protein 44, F box protein FBX30, F box/G domain protein 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82857-2-PBS targets FBXO44 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0977

Product name: Recombinant human FBXO44 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-224 aa of BC007832

Sequence: MAVGNINELPENILLELFTHVPARQLLLNCRLVCSLWRDLIDLVTLWKRKCLREGFITEDWDQPVADWKIFYFLRSLHRNLLHNPCAEEGFEFWSLDVNGGDEWKVEDLSRDQRKEFPNDQVRSQARLRVQVPAVRSAPVVRARASGDLPARPGDHPAEERCQVEGGLPHILQLPARRPLHLVSARRRGHSLLGRLVRPEGHQQQHHHRAPAALTPPEPPSAEP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 F-box protein 44
别名 230229G7, F box only protein 44, F box protein 44, F box protein FBX30, F box/G domain protein 3
计算分子量 26 kDa
观测分子量30 kDa
GenBank蛋白编号BC007832
基因名称 FBXO44
Gene ID (NCBI) 93611
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H4M3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)

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