验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, PC-3 cells |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
24282-1-AP targets FBXO28 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | FBXO28 fusion protein Ag14900 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | F-box protein 28 |
| 别名 | F box only protein 28, F box protein 28, Fbx28, FBXO28, KIAA0483 |
| 计算分子量 | 368 aa, 41 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC031576 |
| 基因名称 | FBXO28 |
| Gene ID (NCBI) | 23219 |
| RRID | AB_2879469 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NVF7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXO28 also named as F box only protein 28 is a 368 amino-acid protein, which contain 1 F-box domain and belongs to F-box protein family. FBXO28 is a critical component of SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. FBOX28 encodes a cell cycle regulated protein with a critical function in tumor cell proliferation. FBXO28 assembles an SCF-FBXO28 ubiquitin ligase whose activity is required during progression through the cell cycle by cyclin-dependent kinase 1/2 mediated phosphorylation. FBXO28 expression and phosphorylation to be strongly associated with poor prognosis and worse overall survival in human breast cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO28 antibody 24282-1-AP | Download protocol |
| IHC protocol for FBXO28 antibody 24282-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
