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验证数据展示

  • IP experiment of HCT 116 using 16505-1-AP

    IP experiment of HCT 116 using 16505-1-AP

    IP result of anti-FBXL8 (IP:16505-1-AP, 4ug; Detection:16505-1-AP 1:500) with HCT 116 cells lysate 1920 ug.

  • IHC staining of mouse heart using 16505-1-AP

    IHC staining of mouse heart using 16505-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16505-1-AP (FBXL8 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FBXL8 Polyclonal antibody

FBXL8 Polyclonal Antibody for IHC, IP, ELISA
Cat No. 16505-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IP, ELISA

别名

FBL8, F-box and leucine-rich repeat protein 8, F-box protein FBL8, F-box/LRR-repeat protein 8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inHCT 116 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16505-1-AP targets FBXL8 in IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 FBXL8 fusion protein Ag9685 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box and leucine-rich repeat protein 8
别名 FBL8, F-box and leucine-rich repeat protein 8, F-box protein FBL8, F-box/LRR-repeat protein 8
计算分子量 374 aa, 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC014414
基因名称 FBXL8
Gene ID (NCBI) 55336
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96CD0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXL8 is a conserved F-box protein that belongs to the ubiquitin ligase complex. It plays a significant role in various cellular processes, including tumorigenesis and metastasis. In colorectal cancer, FBXL8 promotes liver metastasis and stem-cell-like features by mediating ubiquitination and degradation of TP53. Additionally, FBXL8 has been shown to inhibit lymphoma growth and hematopoietic malignancies by targeting specific substrates for degradation. In the context of cardiac fibrosis, FBXL8 inhibits post-myocardial infarction cardiac fibrosis by regulating the ubiquitination of key proteins (PMID: 36855778, PMID: 33122824, PMID: 38615011).

实验方案

Product Specific Protocols
IHC protocol for FBXL8 antibody 16505-1-APDownload protocol
IP protocol for FBXL8 antibody 16505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols