验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20949-1-AP targets FBXL20 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag15071 Product name: Recombinant human FBXL20 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 80-436 aa of BC007557 Sequence: RVVENISKRCGGFLRKLSLRGCLGVGDNALRTFAQNCRNIEVLNLNGCTKTTDATCTSLSKFCSKLRHLDLASCTSITNMSLKALSEGCPLLEQLNISWCDQVTKDGIQALVRGCGGLKALFLKGCTQLEDEALKYIGAHCPELVTLNLQTCLQITDEGLITICRGCHKLQSLCASGCSNITDAILNALGQNCPRLRILEVARCSQLTDVGFTTLARNCHELEKMDLEECVQITDSTLIQLSIHCPRLQVLSLSHCELITDDGIRHLGNGACAHDQLEVIELDNCPLITDASLEHLKSCHSLERIELYDCQQITRAGIKRLRTHLPNIKVHAYFAPVTPPPSVGGSRQRFCRCCIIL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | F-box and leucine-rich repeat protein 20 |
| 别名 | Fbl2, Fbl20 |
| 计算分子量 | 436 aa, 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC007557 |
| 基因名称 | FBXL20 |
| Gene ID (NCBI) | 84961 |
| RRID | AB_3742010 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96IG2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXL20 (F-box and leucine-rich repeat protein 20) is a member of the F-box protein family, which plays a crucial role in the ubiquitin-proteasome system. FBXL20 acts as a substrate recognition component of the SCF (Skp1-Cul1-F-box) ubiquitin ligase complex, targeting specific proteins for ubiquitination and subsequent degradation. This protein is involved in cell cycle regulation, signal transduction, and maintenance of protein homeostasis. Abnormal expression of FBXL20 has been implicated in various diseases, including cancer and neurodegenerative disorders. Research also suggests that FBXL20 may regulate the degradation of 4-1BB, an important immune stimulator, through ubiquitination. (PMID: 25593308, PMID: 27502938, PMID: 35112462)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FBXL20 antibody 20949-1-AP | Download protocol |
| WB protocol for FBXL20 antibody 20949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
