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验证数据展示

  • WB analysis of mouse heart using 83910-5-RR (same clone as 83910-5-PBS)

    WB analysis of mouse heart using 83910-5-RR (same clone as 83910-5-PBS)

    Mouse heart tissue were subjected to SDS PAGE followed by western blot with 83910-5-RR (FBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83910-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 83910-5-RR (same clone as 83910-5-PBS)

    IHC staining of mouse skeletal muscle using 83910-5-RR (same clone as 83910-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83910-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 83910-5-RR (same clone as 83910-5-PBS)

    IHC staining of mouse skeletal muscle using 83910-5-RR (same clone as 83910-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83910-5-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83910-5-RR (same clone as 83910-5-PBS)

    IHC staining of human ovary cancer using 83910-5-RR (same clone as 83910-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83910-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 83910-5-RR (same clone as 83910-5-PBS)

    IF Staining of mouse skeletal muscle using 83910-5-RR (same clone as 83910-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBP2 antibody (83910-5-RR, Clone: 240879C9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83910-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83910-5-RR

    Affinity and Kinetic Characterization of 83910-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83910-5-RR against Human FBP2 were performed. The affinity constant is 4.67 nM.

FBP2 Recombinant monoclonal antibody, PBS Only

FBP2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83910-5-PBS

产品说明书

CloneNo. 240879C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Muscle FBPase, Fructose-1,6-bisphosphatase isozyme 2, FBPase 2, EC:3.1.3.11, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83910-5-PBS targets FBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18245

Product name: Recombinant human FBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 283-339 aa of BC113632

Sequence: NPVAYIIEQAGGLATTGTQPVLDVKPEAIHQRVPLILGSPEDVQEYLTCVQKNQAGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fructose-1,6-bisphosphatase 2
别名 Muscle FBPase, Fructose-1,6-bisphosphatase isozyme 2, FBPase 2, EC:3.1.3.11, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2
计算分子量 339 aa, 37 kDa
观测分子量37 kDa
GenBank蛋白编号BC113632
基因名称 FBP2
Gene ID (NCBI) 8789
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO00757
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FBP2, also named muscle FBP, belongs to the FBPase class 1 family. FBP2 is a ubiquitously expressed enzyme of glycogen synthesis from non-carbohydrates, e.g., lactate (glyconeogenesis). However, it also plays a variety of non-enzymatic functions. FBP2 is involved in the regulation of hypoxia-inducible factor 1 (Hif1) stability, cell cycle-dependent events, biogenesis of mitochondria, and protection of the organelles against high reactive oxygen species (ROS)- and high Ca2+-induced stress (PMID: 32962293, PMID: 35626746). The calculated molecular weight of FBP2 is 37 kDa.

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