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验证数据展示

  • WB analysis of mouse heart using 83910-5-RR

    WB analysis of mouse heart using 83910-5-RR

    Mouse heart tissue were subjected to SDS PAGE followed by western blot with 83910-5-RR (FBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 83910-5-RR

    IHC staining of mouse skeletal muscle using 83910-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 83910-5-RR

    IHC staining of mouse skeletal muscle using 83910-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 83910-5-RR

    IHC staining of human ovary cancer using 83910-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skeletal muscle using 83910-5-RR

    IF Staining of mouse skeletal muscle using 83910-5-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBP2 antibody (83910-5-RR, Clone: 240879C9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83910-5-RR

    Affinity and Kinetic Characterization of 83910-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83910-5-RR against Human FBP2 were performed. The affinity constant is 4.67 nM.

FBP2 Recombinant monoclonal antibody

FBP2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83910-5-RR

产品说明书

CloneNo. 240879C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Muscle FBPase, Fructose-1,6-bisphosphatase isozyme 2, FBPase 2, EC:3.1.3.11, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83910-5-RR targets FBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18245

Product name: Recombinant human FBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 283-339 aa of BC113632

Sequence: NPVAYIIEQAGGLATTGTQPVLDVKPEAIHQRVPLILGSPEDVQEYLTCVQKNQAGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fructose-1,6-bisphosphatase 2
别名 Muscle FBPase, Fructose-1,6-bisphosphatase isozyme 2, FBPase 2, EC:3.1.3.11, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2
计算分子量 339 aa, 37 kDa
观测分子量37 kDa
GenBank蛋白编号BC113632
基因名称 FBP2
Gene ID (NCBI) 8789
RRIDAB_3671492
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO00757
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBP2, also named muscle FBP, belongs to the FBPase class 1 family. FBP2 is a ubiquitously expressed enzyme of glycogen synthesis from non-carbohydrates, e.g., lactate (glyconeogenesis). However, it also plays a variety of non-enzymatic functions. FBP2 is involved in the regulation of hypoxia-inducible factor 1 (Hif1) stability, cell cycle-dependent events, biogenesis of mitochondria, and protection of the organelles against high reactive oxygen species (ROS)- and high Ca2+-induced stress (PMID: 32962293, PMID: 35626746). The calculated molecular weight of FBP2 is 37 kDa.

实验方案

Product Specific Protocols
IF protocol for FBP2 antibody 83910-5-RRDownload protocol
IHC protocol for FBP2 antibody 83910-5-RRDownload protocol
WB protocol for FBP2 antibody 83910-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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