验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, mouse kidney tissue, rat heart tissue |
Positive IHC detected in | human colon cancer tissue, mouse lung tissue, mouse colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29992-1-AP targets Fibulin 5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | Fibulin 5 fusion protein Ag30835 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | fibulin 5 |
别名 | ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, FLJ90059, UP50, Urine p50 protein |
计算分子量 | 448 aa, 50 kDa |
观测分子量 | 65-70 kDa |
GenBank蛋白编号 | BC022280 |
基因名称 | Fibulin 5 |
Gene ID (NCBI) | 10516 |
RRID | AB_2935495 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UBX5 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibulin-5, also known as EVEC and DANCE, is an extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.
实验方案
Product Specific Protocols | |
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WB protocol for Fibulin 5 antibody 29992-1-AP | Download protocol |
IHC protocol for Fibulin 5 antibody 29992-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |