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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated FBF1 Polyclonal antibody

FBF1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-11531

Print datasheet

Synonyms

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, FLJ00103, FLJ13996, HCG1989313, isoform CRA_b, KIAA1863

验证数据展示

  • IF Staining of A549 using CL594-11531

    IF Staining of A549 using CL594-11531

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 FBF1 antibody (CL594-11531) at dilution of 1:200.

经过测试的应用

Positive IF detected inA549 cells, HUVEC cells
For other applications, we recommend the unconjugated version of this antibody, 11531-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-11531 targets FBF1 in IF applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse
Immunogen FBF1 fusion protein Ag2106 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fas (TNFRSF6) binding factor 1
Synonyms Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, FLJ00103, FLJ13996, HCG1989313, isoform CRA_b, KIAA1863
Calculated Molecular Weight1133 aa, 125 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC023549
Gene Symbol FBF1
Gene ID (NCBI) 85302
RRIDAB_2919767
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TES7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.

实验方案

Product Specific Protocols
IF protocol for CL594 FBF1 antibody CL594-11531Download protocol
Standard Protocols
Click here to view our Standard Protocols