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验证数据展示

  • WB analysis using 30190-1-AP (same clone as 30190-1-PBS)

    WB analysis using 30190-1-AP (same clone as 30190-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30190-1-AP (FATS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30190-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 30190-1-AP (same clone as 30190-1-PBS)

    IF Staining of A431 using 30190-1-AP (same clone as 30190-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FATS antibody (30190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30190-1-PBS in a different storage buffer formulation.

FATS Polyclonal antibody, PBS Only

FATS Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30190-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30190-1-PBS targets FATS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32956

Product name: Recombinant human C10orf90 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 169-400 aa of NM_001004298.2

Sequence: AEDTLFQAPPALANGAHPGRHQRSFACTEFSRNSSVVRLKVPEAHTGLCERRKYWVTHADDKETSFSPDTPLSGKSPLVFSSCVHLRVSQQCPDSIYYVDKSLSVPIEPPQIASPKMHRSVLSLNLNCSSHRLTADGVDGLVNREPISEALKQELLEGDQDLVGQRWNPGLQESHLKETPSLRRVHLGTGACPWSGSFPLENTELANVGANQVTVRKGEKDHTTHCHASDHA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 90
别名
计算分子量78 kDa
观测分子量 67-77 kDa
GenBank蛋白编号NM_001004298.2
基因名称 FATS/C10orf90
Gene ID (NCBI) 118611
RRIDAB_2935528
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96M02
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FATS, also known as centrosomal protein C10orf90, bA422P15.2, belongs to a novel class of enzymes that acts as an E2-E3 hybrid enzyme for assembly of polyubiquitin chains. FATS is an E2-independent ubiquitin ligase that stabilizes p53 and promotes its activation in response to DNA damage (PMID: 24240685). FATS acts as a p53/TP53 activator by inhibiting MDM2 binding to p53/TP53 and stimulating non-proteolytic polyubiquitination of p53/TP53.

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