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验证数据展示

  • WB analysis using 30190-1-AP

    WB analysis using 30190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30190-1-AP (FATS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30190-1-AP

    IF Staining of A431 using 30190-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FATS antibody (30190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

FATS Polyclonal antibody

FATS Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30190-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

bA422P15.2, C10orf90, FATS, FLJ32938

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, mouse liver tissue, rat brain tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30190-1-AP targets FATS in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 FATS fusion protein Ag32956 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 90
别名 bA422P15.2, C10orf90, FATS, FLJ32938
计算分子量78 kDa
观测分子量 67-77 kDa
GenBank蛋白编号NM_001004298.2
基因名称 FATS/C10orf90
Gene ID (NCBI) 118611
RRIDAB_2935528
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96M02
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FATS, also known as centrosomal protein C10orf90, bA422P15.2, belongs to a novel class of enzymes that acts as an E2-E3 hybrid enzyme for assembly of polyubiquitin chains. FATS is an E2-independent ubiquitin ligase that stabilizes p53 and promotes its activation in response to DNA damage (PMID: 24240685). FATS acts as a p53/TP53 activator by inhibiting MDM2 binding to p53/TP53 and stimulating non-proteolytic polyubiquitination of p53/TP53.

实验方案

Product Specific Protocols
WB protocol for FATS antibody 30190-1-APDownload protocol
IF protocol for FATS antibody 30190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols