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验证数据展示

  • WB analysis of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    WB analysis of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    WB result of FASN antibody (66591-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASN transfected HeLa cells. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

    IHC staining of human breast cancer using 66591-1-Ig (same clone as 66591-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66591-1-Ig (FASN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FASN antibody included
  • WB analysis of HepG2 using 66591-1-Ig (same clone as 66591-1-PBS)

    WB analysis of HepG2 using 66591-1-Ig (same clone as 66591-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66591-1-Ig (FASN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51570-1

    Cytometric bead array sample test of MP51570-1

    Cytometric bead array sample test of MP51570-1, FASN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66591-2-PBS. Detection antibody: 66591-1-PBS.

  • IF Staining of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    IF Staining of HeLa using 66591-1-Ig (same clone as 66591-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (66591-1-Ig, Clone: 1B6E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66591-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51570-1

    Cytometric bead array standard curve of MP51570-1

    Cytometric bead array standard curve of MP51570-1, FASN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66591-2-PBS. Detection antibody: 66591-1-PBS. Standard:Ag0975. Range: 0.781-100 ng/mL.

  • Featured Product
  • KD/KO验证

FASN Monoclonal antibody, PBS Only (Detector)

FASN Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66591-1-PBS

产品说明书

CloneNo. 1B6E4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

1B6E4, FAS, fatty acid synthase, Type I fatty acid synthase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FASN Ready-To-Use IHC Kit. FASN primary antibody included.

产品信息

66591-1-PBS targets FASN as part of a matched antibody pair:

MP51570-1: 66591-2-PBS capture and 66591-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0975

Product name: Recombinant human FASN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 142-439 aa of BC007909

Sequence: QTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fatty acid synthase
别名 1B6E4, FAS, fatty acid synthase, Type I fatty acid synthase
计算分子量 272 kDa
观测分子量 272 kDa
GenBank蛋白编号BC007909
基因名称 FASN
Gene ID (NCBI) 2194
RRIDAB_2881951
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP49327
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

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