FASN Monoclonal antibody
FASN Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (3)
Applications
IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B6E4
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HEK-293 cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy FASN Ready-To-Use IHC Kit. FASN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66591-1-Ig targets FASN in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, monkey |
| Immunogen | FASN fusion protein Ag0975 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | fatty acid synthase |
| Synonyms | FAS, FASN, fatty acid synthase, OA 519, SDR27X1 |
| Calculated Molecular Weight | 272 kDa |
| Observed Molecular Weight | 272 kDa |
| GenBank Accession Number | BC007909 |
| Gene Symbol | FASN |
| Gene ID (NCBI) | 2194 |
| RRID | AB_2881951 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P49327 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FASN antibody 66591-1-Ig | Download protocol |
| IHC protocol for FASN antibody 66591-1-Ig | Download protocol |
| IF protocol for FASN antibody 66591-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. | ||
Theranostics Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription. | ||
Am J Cancer Res Ubiquitin specific peptidase 38 promotes the progression of gastric cancer through upregulation of fatty acid synthase. | ||
Environ Toxicol SIRT1/mTOR pathway-mediated autophagy dysregulation promotes Pb-induced hepatic lipid accumulation in HepG2 cells | ||
Front Oncol CRISPR Cas9-mediated ablation of pyruvate carboxylase gene in colon cancer cell line HT-29 inhibits growth and migration, induces apoptosis and increases sensitivity to 5-fluorouracil and glutaminase inhibitor |
