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验证数据展示

  • WB analysis using 10624-2-AP (same clone as 10624-2-PBS)

    WB analysis using 10624-2-AP (same clone as 10624-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10624-2-AP (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 10624-2-AP (same clone as 10624-2-PBS)

    WB analysis of HeLa using 10624-2-AP (same clone as 10624-2-PBS)

    WB result of FASN antibody (10624-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASN transfected HeLa cells. This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 10624-2-AP (same clone as 10624-2-PBS)

    FC experiment of HeLa using 10624-2-AP (same clone as 10624-2-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human FASN (10624-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10624-2-AP (same clone as 10624-2-PBS)

    IHC staining of human breast cancer using 10624-2-AP (same clone as 10624-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FASN antibody included
  • IHC staining of mouse brown adipose using 10624-2-AP (same clone as 10624-2-PBS)

    IHC staining of mouse brown adipose using 10624-2-AP (same clone as 10624-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FASN antibody included
  • IHC staining of rat brown adipose using 10624-2-AP (same clone as 10624-2-PBS)

    IHC staining of rat brown adipose using 10624-2-AP (same clone as 10624-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FASN antibody included
  • IP experiment of mouse liver using 10624-2-AP (same clone as 10624-2-PBS)

    IP experiment of mouse liver using 10624-2-AP (same clone as 10624-2-PBS)

    IP result of anti-FASN (IP:10624-2-AP, 4ug; Detection:10624-2-AP 1:10000) with mouse liver tissue lysate 2400 ug. This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 10624-2-AP (same clone as 10624-2-PBS)

    IHC staining of mouse brain using 10624-2-AP (same clone as 10624-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FASN antibody included
  • IF Staining of HeLa using 10624-2-AP (same clone as 10624-2-PBS)

    IF Staining of HeLa using 10624-2-AP (same clone as 10624-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (10624-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FASN Polyclonal antibody, PBS Only

FASN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10624-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

FAS, fatty acid synthase, Type I fatty acid synthase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FASN Ready-To-Use IHC Kit. FASN primary antibody included.

产品信息

10624-2-PBS targets FASN in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0975

Product name: Recombinant human FASN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 142-439 aa of BC007909

Sequence: QTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fatty acid synthase
别名 FAS, fatty acid synthase, Type I fatty acid synthase
计算分子量 272 kDa
观测分子量 250-272 kDa
GenBank蛋白编号BC007909
基因名称 FASN
Gene ID (NCBI) 2194
RRIDAB_2100801
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49327
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

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