验证数据展示

WB analysis using 10624-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10624-2-AP (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10624-2-AP
WB result of FASN antibody (10624-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASN transfected HeLa cells.
FC experiment of HeLa using 10624-2-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human FASN (10624-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
IHC staining of human breast cancer using 10624-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with FASN antibody included
IHC staining of mouse brown adipose using 10624-2-AP
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with FASN antibody included
IHC staining of rat brown adipose using 10624-2-AP
Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with FASN antibody included
IP experiment of mouse liver using 10624-2-AP
IP result of anti-FASN (IP:10624-2-AP, 4ug; Detection:10624-2-AP 1:10000) with mouse liver tissue lysate 2400 ug.
IHC staining of mouse brain using 10624-2-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with FASN antibody included
IF Staining of HeLa using 10624-2-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (10624-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO验证

FASN Polyclonal antibody

FASN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10624-2-AP

产品说明书

引用文献(284)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (7)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

FAS, fatty acid synthase, Type I fatty acid synthase

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (284)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, SH-SY5Y cells, mouse liver tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human breast cancer tissue, mouse brain tissue, mouse brown adipose tissue, rat brown adipose tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy FASN Ready-To-Use IHC Kit. FASN primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 6 publications below
WB	See 257 publications below
IHC	See 37 publications below
IF	See 15 publications below
IP	See 5 publications below
CoIP	See 1 publications below

产品信息

10624-2-AP targets FASN in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, chicken, zebrafish, bovine, sheep, goat, duck
免疫原	CatNo: Ag0975 Product name: Recombinant human FASN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 142-439 aa of BC007909 Sequence: QTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	fatty acid synthase
别名	FAS, fatty acid synthase, Type I fatty acid synthase
计算分子量	272 kDa
观测分子量	250-272 kDa
GenBank蛋白编号	BC007909
基因名称	FASN
Gene ID (NCBI)	2194
RRID	AB_2100801
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P49327
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

实验方案

Product Specific Protocols
IF protocol for FASN antibody 10624-2-AP	Download protocol
IHC protocol for FASN antibody 10624-2-AP	Download protocol
IP protocol for FASN antibody 10624-2-AP	Download protocol
WB protocol for FASN antibody 10624-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate Authors - Peng Zhou View Article
human	IHC	Nat Cancer Integrative proteogenomic profiling of high-risk prostate cancer samples from Chinese patients indicates metabolic vulnerabilities and diagnostic biomarkers Authors - Baijun Dong View Article
mouse	WB	Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. Authors - Yi Fu View Article
mouse	WB	Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. Authors - Dong Huang View Article
mouse	IF	Nat Commun A nanoemulsion targeting adipose hypertrophy and hyperplasia shows anti-obesity efficiency in female mice Authors - Yichao Lu View Article
mouse	WB	Nat Commun Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity Authors - Jinjie Duan View Article

验证数据展示

WB analysis using 10624-2-AP

WB analysis of HeLa using 10624-2-AP

FC experiment of HeLa using 10624-2-AP

IHC staining of human breast cancer using 10624-2-AP

IHC staining of mouse brown adipose using 10624-2-AP

IHC staining of rat brown adipose using 10624-2-AP

IP experiment of mouse liver using 10624-2-AP

IHC staining of mouse brain using 10624-2-AP

IF Staining of HeLa using 10624-2-AP

FASN Polyclonal antibody

FASN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate

Integrative proteogenomic profiling of high-risk prostate cancer samples from Chinese patients indicates metabolic vulnerabilities and diagnostic biomarkers

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

A nanoemulsion targeting adipose hypertrophy and hyperplasia shows anti-obesity efficiency in female mice

Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity