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CoraLite® Plus 488-conjugated FARSA Monoclonal antibody

FARSA Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D3E2

Cat No : CL488-67945

Print datasheet

Synonyms

CML33, FARS, FARSA, FARSL, FARSLA, FRSA, PheHA, PheRS

验证数据展示

  • FC experiment of K-562 using CL488-67945

    FC experiment of K-562 using CL488-67945

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FARSA (CL488-67945, Clone:2D3E2) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy FARSA Ready-To-Use IHC Kit. FARSA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67945-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67945 targets FARSA in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat
Immunogen FARSA fusion protein Ag12647 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name phenylalanyl-tRNA synthetase, alpha subunit
Synonyms CML33, FARS, FARSA, FARSL, FARSLA, FRSA, PheHA, PheRS
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC006495
Gene Symbol FARSA
Gene ID (NCBI) 2193
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y285
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 FARSA antibody CL488-67945Download protocol
Standard Protocols
Click here to view our Standard Protocols