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验证数据展示

  • WB analysis using 20108-1-AP (same clone as 20108-1-PBS)

    WB analysis using 20108-1-AP (same clone as 20108-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20108-1-AP (FAM96B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 20108-1-AP (same clone as 20108-1-PBS)

    IHC staining of mouse kidney using 20108-1-AP (same clone as 20108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20108-1-AP (FAM96B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 20108-1-AP (same clone as 20108-1-PBS)

    IP experiment of K-562 using 20108-1-AP (same clone as 20108-1-PBS)

    IP result of anti-FAM96B (IP:20108-1-AP, 4ug; Detection:20108-1-AP 1:300) with K-562 cells lysate 2400 ug. This data was developed using the same antibody clone with 20108-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FAM96B Polyclonal antibody, PBS Only

FAM96B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 20108-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20108-1-PBS targets FAM96B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13827

Product name: Recombinant human FAM96B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-163 aa of BC001733

Sequence: MVGGGGVGGGLLENANPLIYQRSGERPVTAGEEDEQVPDSIDAREIFDLIRSINDPEHPLTLEELNVVEQVRVQVSDPESTVAVAFTPTIPHCSMATLIGLSIKVKLLRSLPQRFKMDVHITPGTHASEHAVNKQLADKERVAAALENTHLLEVVNQCLSARS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 96, member B
别名
计算分子量 163 aa, 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC001733
基因名称 FAM96B
Gene ID (NCBI) 51647
RRIDAB_10666848
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3D0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAM96B, also known as MIP18 or CIAO2B (Cytosolic iron-sulfur assembly component 2B), is a component of the cytosolic iron-sulfur protein assembly (CIA) complex involved in iron homeostasis and part of the MMXD complex contributed to proper chromosome segregation (PMID: 36503010). FAM96B may act as a scaffold protein to recruit CKB and the other components of the MMXD complex to associate with the spindle and mitotic apparatus and contributes to chromosome segregation.

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