- Featured Product
- KD/KO Validated
FAM96B Polyclonal antibody
FAM96B Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Ramos cells, HeLa cells, K-562 cells |
Positive IHC detected in | mouse kidney tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 9 publications below |
CoIP | See 1 publications below |
产品信息
20108-1-AP targets FAM96B in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, ELISA |
Cited Applications | WB, CoIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | FAM96B fusion protein Ag13827 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | family with sequence similarity 96, member B |
Synonyms | CGI 128, CIAB, CIAO2B, FAM96B, HSPC118, MIP18, Protein FAM96B |
Calculated Molecular Weight | 163 aa, 18 kDa |
Observed Molecular Weight | 18 kDa |
GenBank Accession Number | BC001733 |
Gene Symbol | FAM96B |
Gene ID (NCBI) | 51647 |
RRID | AB_10666848 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y3D0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for FAM96B antibody 20108-1-AP | Download protocol |
IHC protocol for FAM96B antibody 20108-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Science MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.
| ||
Nat Struct Mol Biol Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators. | ||
Cell Metab Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins.
| ||
Mol Cell An Oxygen-Dependent Interaction between FBXL5 and the CIA-Targeting Complex Regulates Iron Homeostasis. | ||
Cell Rep The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins. | ||
J Biol Chem The Association of the DNA helicase XPD with Transcription Factor IIH is regulated by the Cytosolic Iron-sulfur Cluster Assembly Pathway. |