验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24803-1-AP targets FAM92A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag20738 Product name: Recombinant human FAM92A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 162-318 aa of BC014598 Sequence: ATLTARNREAKQLTQLERTRQRNPSDRHVISQAETELQRAAMDASRTSRHLEETINNFERQKMKDIKTIFSEFITIEMLFHGKALEVYTAAYQNIQNIDEDEDLEVFRNSLYAPDYSSRLDIVRANSKSPLQRSLSAKCVSGTGQEAGWAATCQTLI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 92, member A1 |
| 别名 | CIBAR1, FAM92A, CBY1-interacting BAR domain-containing protein 1 |
| 计算分子量 | 289 aa, 33 kDa |
| 观测分子量 | 29-33 kDa |
| GenBank蛋白编号 | BC014598 |
| 基因名称 | FAM92A1 |
| Gene ID (NCBI) | 137392 |
| RRID | AB_2879735 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A1XBS5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM92A1 antibody 24803-1-AP | Download protocol |
| IHC protocol for FAM92A1 antibody 24803-1-AP | Download protocol |
| IP protocol for FAM92A1 antibody 24803-1-AP | Download protocol |
| WB protocol for FAM92A1 antibody 24803-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Membrane remodeling by FAM92A1 during brain development regulates neuronal morphology, synaptic function, and cognition | ||
J Exp Clin Cancer Res Nuclear FAM289-Galectin-1 interaction controls FAM289-mediated tumor promotion in malignant glioma. | ||
Adv Sci (Weinh) The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers | ||
Dev Cell A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biology. | ||
Am J Hum Genet Mutations in SCNM1 cause orofaciodigital syndrome due to minor intron splicing defects affecting primary cilia | ||
Life Sci Alliance ciBAR1 loss in mice causes laterality defects, pancreatic degeneration, and altered glucose tolerance |
