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验证数据展示

  • WB analysis of human milk using 27131-1-AP

    WB analysis of human milk using 27131-1-AP

    human milk were subjected to SDS PAGE followed by western blot with 27131-1-AP (FAM3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 27131-1-AP

    IHC staining of human pancreas using 27131-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27131-1-AP (FAM3B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 27131-1-AP

    IHC staining of human pancreas using 27131-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27131-1-AP (FAM3B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM3B Polyclonal antibody

FAM3B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27131-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C21orf11, C21orf76, Cytokine like protein 2 21, FAM3B, ORF9, Pancreatic derived factor, PANDER, PRED44, Protein FAM3B, UNQ320/PRO365

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman milk tissue
Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

27131-1-AP targets FAM3B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag25955

Product name: Recombinant human FAM3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 60-235 aa of BC057829

Sequence: RQKCDHWTPCPSDTYAYRLLSGGGRSKYAKICFEDNLLMGEQLGNVARGINIAIVNYVTGNVTATRCFDMYEGDNSGPMTKFIQSAAPKSLLFMVTYDDGSTRLNNDAKNAIEALGSKEIRNMKFRSSWVFIAAKGLELPSEIQREKINHSDAKNNRYSGWPAEIQIEGCIPKERS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 3, member B
别名 C21orf11, C21orf76, Cytokine like protein 2 21, FAM3B, ORF9, Pancreatic derived factor, PANDER, PRED44, Protein FAM3B, UNQ320/PRO365
计算分子量 26 kDa
观测分子量 26 kDa, 23 kDa
GenBank蛋白编号BC057829
基因名称 FAM3B
Gene ID (NCBI) 54097
RRIDAB_2880766
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP58499
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM3B, also known as Pancreatic-derived factor (PANDER), is a secreted glycoprotein that belongs to the cytokine like-protein family. There are four members in this gene family (FAM3A, FAM3B, FAM3C and FAM3D). As a result of alternative splicing and alternate signal peptide cleavage sites, multiple isoforms are known. FAM3B is selectively expressed at a high level in pancreatic islets and at low levels in the small intestine, prostate, and certain neurons.

实验方案

Product Specific Protocols
WB protocol for FAM3B antibody 27131-1-APDownload protocol
IHC protocol for FAM3B antibody 27131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Int J Gen Med

FAM3B Serves as a Biomarker for the Development and Malignancy of Oral Lichen Planus.

Authors - Wenfang Wang
View Article
humanIHC

Front Pharmacol

A senescence-associated signature refines the classification of different modification patterns and characterization of tumor immune microenvironment infiltration in triple-negative breast cancer

Authors - Renhong Huang
View Article