验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells |
| Positive IHC detected in | human prostate cancer tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25752-1-AP targets COX20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag22520 Product name: Recombinant human FAM36A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-118 aa of BC018519 Sequence: MAAPPEPGEPEERKSLKLLGFLDVENTPCARHSILYGSLGSVVAGFGHFLFTSRIRRSCDVGVGGFILVTLGCWFHCRYNYAKQRIQERIAREEIKKKILYEGTHLDPERKHNGSSSN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 36, member A |
| 别名 | FAM36A, Cytochrome c oxidase assembly protein COX20, mitochondrial, FAM 36A |
| 计算分子量 | 118 aa, 13 kDa |
| 观测分子量 | ~16 kDa |
| GenBank蛋白编号 | BC018519 |
| 基因名称 | COX20 |
| Gene ID (NCBI) | 116228 |
| RRID | AB_2880224 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5RI15 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COX20, also name as FAM36A, belongs to the COX20 family. Mutations in COX20 are a novel cause of recessively inherited.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for COX20 antibody 25752-1-AP | Download protocol |
| IHC protocol for COX20 antibody 25752-1-AP | Download protocol |
| WB protocol for COX20 antibody 25752-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers. | ||
Sci Transl Med NIT2 dampens BRD1 phase separation and restrains oxidative phosphorylation to enhance chemosensitivity in gastric cancer | ||
Sci Data Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium. | ||
Front Endocrinol (Lausanne) Novel prognostic features and personalized treatment strategies for mitochondria-related genes in glioma patients |
