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验证数据展示

  • WB analysis using 33047-1-AP (same clone as 33047-1-PBS)

    WB analysis using 33047-1-AP (same clone as 33047-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33047-1-AP (FAM21C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33047-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 33047-1-AP (same clone as 33047-1-PBS)

    IF Staining of A431 using 33047-1-AP (same clone as 33047-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FAM21C antibody (33047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33047-1-PBS in a different storage buffer formulation.

FAM21C Polyclonal antibody, PBS Only

FAM21C Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 33047-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

KIAA0592, Vaccinia virus penetration factor, VPEF, WASH complex subunit 2C, WASHC2C

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33047-1-PBS targets FAM21C in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15066

Product name: Recombinant human FAM21C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1036-1318 aa of BC006456

Sequence: MSVPRGPIAQWADGAISPNGHRPQLRAASGEDSTEEALAAAAAPWEGGPVPGVDTSPFAKSLGHSRGEADLFDSGDIFSTGTGSQSVERTKPKAKIAENPANPPVGGKAKSPMFPALGEASSDDDLFQSAKPKPAKKTNPFPLLEDEDDLFTDQKVKKNETKSSSQQDVILTTQDIFEDDIFATEAIKPSQKTREKEKTLESNLFDDNIDIFADLTVKPKEKSKKKVEAKSIFDDDMDDIFSTGIQAKTTKPKSRSAQAAPEPRFEHKVSNIFDDPLNAFGGQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 21, member C
别名 KIAA0592, Vaccinia virus penetration factor, VPEF, WASH complex subunit 2C, WASHC2C
计算分子量 1320 aa, 145 kDa
观测分子量200 kDa
GenBank蛋白编号BC006456
基因名称 FAM21C
Gene ID (NCBI) 253725
RRIDAB_3742836
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Y4E1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WASHC2C encodes WASH complex subunit 2C, a core subunit of the WASH complex that organizes endosomal membrane remodeling and cargo recycling. It binds phospholipids such as PtdIns-(4)P and the retromer component VPS35, linking endosomal localization to actin-based membrane dynamics. The protein is found on the early endosome membrane, in the early endosome, endosome, and cytosol, and is expressed at high levels in the male reproductive system with detectable expression in brain, endocrine system, and hematopoietic system.

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