验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, HuH-7 cells, L02 cells |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33047-1-AP targets FAM21C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag15066 Product name: Recombinant human FAM21C protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1036-1318 aa of BC006456 Sequence: MSVPRGPIAQWADGAISPNGHRPQLRAASGEDSTEEALAAAAAPWEGGPVPGVDTSPFAKSLGHSRGEADLFDSGDIFSTGTGSQSVERTKPKAKIAENPANPPVGGKAKSPMFPALGEASSDDDLFQSAKPKPAKKTNPFPLLEDEDDLFTDQKVKKNETKSSSQQDVILTTQDIFEDDIFATEAIKPSQKTREKEKTLESNLFDDNIDIFADLTVKPKEKSKKKVEAKSIFDDDMDDIFSTGIQAKTTKPKSRSAQAAPEPRFEHKVSNIFDDPLNAFGGQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 21, member C |
| 别名 | KIAA0592, Vaccinia virus penetration factor, VPEF, WASH complex subunit 2C, WASHC2C |
| 计算分子量 | 1320 aa, 145 kDa |
| 观测分子量 | 200 kDa |
| GenBank蛋白编号 | BC006456 |
| 基因名称 | FAM21C |
| Gene ID (NCBI) | 253725 |
| RRID | AB_3742836 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9Y4E1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WASHC2C encodes WASH complex subunit 2C, a core subunit of the WASH complex that organizes endosomal membrane remodeling and cargo recycling. It binds phospholipids such as PtdIns-(4)P and the retromer component VPS35, linking endosomal localization to actin-based membrane dynamics. The protein is found on the early endosome membrane, in the early endosome, endosome, and cytosol, and is expressed at high levels in the male reproductive system with detectable expression in brain, endocrine system, and hematopoietic system.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM21C antibody 33047-1-AP | Download protocol |
| WB protocol for FAM21C antibody 33047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
