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验证数据展示

  • WB analysis using 24732-1-AP

    WB analysis using 24732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24732-1-AP (FAM172A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 24732-1-AP

    IHC staining of human liver using 24732-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24732-1-AP (FAM172A Antibody) at dilution of 1:200 (under 40x lens).

FAM172A Polyclonal antibody

FAM172A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24732-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C5orf21, FAM172A, Protein FAM172A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse colon tissue, rat colon tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24732-1-AP targets FAM172A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FAM172A fusion protein Ag20437 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 172, member A
别名 C5orf21, FAM172A, Protein FAM172A
计算分子量 416 aa, 48 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC020584
基因名称 FAM172A
Gene ID (NCBI) 83989
RRIDAB_2923572
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WUF8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Family with sequence similarity 172 member A (FAM172A), cloned from human aortic tissues, is subsequently observed in human endothelial and vascular smooth muscle cells, and macrophages. As the imbalance of the cell cycle and apoptosis is closely related to the key characteristics of malignant tumors, FAM172A dysregulation is a potential contributing factor in cell transformation. Recently, FAM172A has been reported to take part in the development of several cancers, such as human liver, colorectal and papillary thyroid cancers. Moreover, FAM172A was upregulated by high glucose in a concentration- and time-dependent manner which indicated that FAM172A may be involved in the pathogenesis of diabetic related diseases. FAM172A has 4 isoforms with the molecular mass of 34-36, 43 and 48 kDa. This antibody was detected at 50-55 kDa on SDS-PAGE. (PMID: 31988090, 26637224)

实验方案

Product Specific Protocols
WB protocol for FAM172A antibody 24732-1-APDownload protocol
IHC protocol for FAM172A antibody 24732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols