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验证数据展示

  • WB analysis of HeLa using 20520-1-AP

    WB analysis of HeLa using 20520-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20520-1-AP (FAM164C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20520-1-AP

    IHC staining of human kidney using 20520-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 20520-1-AP

    IHC staining of human testis using 20520-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20520-1-AP (FAM164C Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of C6 using 20520-1-AP

    IF Staining of C6 using 20520-1-AP

    Immunofluorescent analysis of C6 cells using 20520-1-AP (FAM164C antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

FAM164C Polyclonal antibody

FAM164C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20520-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Zinc finger C2HC domain-containing protein 1C, ZC2HC1C, C14orf140

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, C6 cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC6 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20520-1-AP targets FAM164C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag14359

Product name: Recombinant human FAM164C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-275 aa of BC004259

Sequence: MAGLQRLASHLPVGVMLPHNTTEAPGPHSAKQDSYEQGDSSQQSLKGHLRNNFQKQLLSNKELILDKVYTHPKWNTQTKARSYSYPHCTGISQQDPESDSQGQGNGLFYSSGPQSWYPKANNQDFIPFTKKRVGVDRAFPLKPMVHRKSCSTGEAGTDGDHNVYPRPPEPREFSSRNFGVRNQGNFSVVGTVLAATQAEKAVANFDRTEWVQIRRLEAAGESLEEEIRRKQILLRGKLKKTEEELRRIQTQKEQAKENENGELQKIILPRSRVKG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 164, member C
别名 Zinc finger C2HC domain-containing protein 1C, ZC2HC1C, C14orf140
计算分子量 275 aa, 31 kDa
观测分子量 31-36 kDa
GenBank蛋白编号BC004259
基因名称 FAM164C
Gene ID (NCBI) 79696
RRIDAB_2878697
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53FD0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM164C, also named as ZC2HC1C or C14orf140, is a 456 amino acid protein, which contains one C2HC-type zinc finger and belongs to the ZC2HC1 family. FAM164C exists as two isoforms. The isoform1 is a 51kDa protein ad isoform2 is 31kDa.

实验方案

Product Specific Protocols
WB protocol for FAM164C antibody 20520-1-APDownload protocol
IHC protocol for FAM164C antibody 20520-1-APDownload protocol
IF protocol for FAM164C antibody 20520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols