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验证数据展示

  • WB analysis of PC-3 using 25388-1-AP

    WB analysis of PC-3 using 25388-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25388-1-AP (FAM160B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 25388-1-AP

    IP experiment of PC-3 using 25388-1-AP

    IP result of anti-FAM160B1 (IP:25388-1-AP, 5ug; Detection:25388-1-AP 1:300) with PC-3 cells lysate 1500ug.

  • IF Staining of PC-3 using 25388-1-AP

    IF Staining of PC-3 using 25388-1-AP

    Immunofluorescent analysis of PC-3 cells using 25388-1-AP (FAM160B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human intrahepatic cholangiocarcinoma using 25388-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 25388-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25388-1-AP (FAM160B1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM160B1 Polyclonal antibody

FAM160B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25388-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

FHIP2A, FHF complex subunit HOOK interacting protein 2A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25388-1-AP targets FAM160B1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22051

Product name: Recombinant human FAM160B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 136-319 aa of BC037207

Sequence: IRLCGEVLATPTENEEIQFLCIVCAKLKQDPYLVNFFLENKMKSLASKGVPNVISEDTLKGQDSLSTDTGQSRQPEELSGATGMEQTELEDEPPHQMDHLSTSLDNLSVTSLPEASVVCPNQDYNLVNSLLNLTRSPDGRIAVKACEGLMLLVSLPEPAAAKCLTQSTCLCELLTDRLASLYKA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 160, member B1
别名 FHIP2A, FHF complex subunit HOOK interacting protein 2A
计算分子量 765 aa, 86 kDa
观测分子量 105 kDa
GenBank蛋白编号BC037207
基因名称 FAM160B1
Gene ID (NCBI) 57700
RRIDAB_2880054
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5W0V3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM160B1, also named as KIAA1600, is a 765 amino acid protein, which belongs to the UPF0518 family. The biological function of FAM160B1is still non-known. FAM160B1 exists as two isoforms and the molecular weight of isoform one is a 86 kDa protein.The isoforms two is produced by alternative splicing and is a 83 kDa protein. There are modified residues of FAM160B1 protein, so we detected 95 kDa and 100 kDa two bands by western blotting.

实验方案

Product Specific Protocols
WB protocol for FAM160B1 antibody 25388-1-APDownload protocol
IHC protocol for FAM160B1 antibody 25388-1-APDownload protocol
IF protocol for FAM160B1 antibody 25388-1-APDownload protocol
IP protocol for FAM160B1 antibody 25388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols