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FAM160B1 Polyclonal antibody

FAM160B1 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 25388-1-AP

Print datasheet

Synonyms

FAM160B1, KIAA1600, Protein FAM160B1

验证数据展示

  • WB analysis of PC-3 using 25388-1-AP

    WB analysis of PC-3 using 25388-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25388-1-AP (FAM160B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 25388-1-AP

    IP experiment of PC-3 using 25388-1-AP

    IP result of anti-FAM160B1 (IP:25388-1-AP, 5ug; Detection:25388-1-AP 1:300) with PC-3 cells lysate 1500ug.

  • IF Staining of PC-3 using 25388-1-AP

    IF Staining of PC-3 using 25388-1-AP

    Immunofluorescent analysis of PC-3 cells using 25388-1-AP (FAM160B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells
Positive IP detected inPC-3 cells
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25388-1-AP is FAM160B1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IP, WB,ELISA
Tested Reactivity human
Immunogen FAM160B1 fusion protein Ag22051 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 160, member B1
Synonyms FAM160B1, KIAA1600, Protein FAM160B1
Calculated Molecular Weight 765 aa, 86 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC037207
Gene Symbol FAM160B1
Gene ID (NCBI) 57700
RRIDAB_2880054
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5W0V3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM160B1, also named as KIAA1600, is a 765 amino acid protein, which belongs to the UPF0518 family. The biological function of FAM160B1is still non-known. FAM160B1 exists as two isoforms and the molecular weight of isoform one is a 86 kDa protein.The isoforms two is produced by alternative splicing and is a 83 kDa protein. There are modified residues of FAM160B1 protein, so we detected 95 kDa and 100 kDa two bands by western blotting.

实验方案

Product Specific Protocols
WB protocol for FAM160B1 antibody 25388-1-APDownload protocol
IF protocol for FAM160B1 antibody 25388-1-APDownload protocol
IP protocol for FAM160B1 antibody 25388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols