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验证数据展示

  • WB analysis of PC-3 using 26498-1-AP

    WB analysis of PC-3 using 26498-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26498-1-AP (FAM131B Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 26498-1-AP

    IHC staining of human brain using 26498-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26498-1-AP (FAM131B Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 26498-1-AP

    IHC staining of human brain using 26498-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26498-1-AP (FAM131B Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of SH-SY5Y using 26498-1-AP

    IF Staining of SH-SY5Y using 26498-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using FAM131B antibody (26498-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FAM131B Polyclonal antibody

FAM131B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26498-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Protein FAM131B, KIAA0773

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, SH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26498-1-AP targets FAM131B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 FAM131B fusion protein Ag24143 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 131, member B
别名 Protein FAM131B, KIAA0773
观测分子量 35-40 kDa
GenBank蛋白编号BC045611
基因名称 FAM131B
Gene ID (NCBI) 9715
RRIDAB_2880534
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86XD5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM131B, also named as KIAA0773, has some isoforms with MW 35-40 kDa.

实验方案

Product Specific Protocols
WB protocol for FAM131B antibody 26498-1-APDownload protocol
IHC protocol for FAM131B antibody 26498-1-APDownload protocol
IF protocol for FAM131B antibody 26498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols