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FAM131B Polyclonal antibody

FAM131B Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 26498-1-AP

Print datasheet

Synonyms

FAM131B, KIAA0773, Protein FAM131B

验证数据展示

  • WB analysis of PC-3 using 26498-1-AP

    WB analysis of PC-3 using 26498-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26498-1-AP (FAM131B Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 26498-1-AP

    IHC staining of human brain using 26498-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26498-1-AP (FAM131B Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 26498-1-AP

    IHC staining of human brain using 26498-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26498-1-AP (FAM131B Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of SH-SY5Y using 26498-1-AP

    IF Staining of SH-SY5Y using 26498-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 26498-1-AP (FAM131B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells, SH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26498-1-AP is FAM131B Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human
Immunogen FAM131B fusion protein Ag24143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 131, member B
Synonyms FAM131B, KIAA0773, Protein FAM131B
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC045611
Gene Symbol FAM131B
Gene ID (NCBI) 9715
RRIDAB_2880534
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86XD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM131B, also named as KIAA0773, has some isoforms with MW 35-40 kDa.

实验方案

Product Specific Protocols
WB protocol for FAM131B antibody 26498-1-APDownload protocol
IHC protocol for FAM131B antibody 26498-1-APDownload protocol
IF protocol for FAM131B antibody 26498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols