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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84619-5-RR (same clone as 84619-5-PBS)

    WB analysis using 84619-5-RR (same clone as 84619-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84619-5-RR (FADD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84619-5-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84619-5-RR (same clone as 84619-5-PBS)

    FC experiment of A431 using 84619-5-RR (same clone as 84619-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug FADD Recombinant antibody (84619-5-RR, Clone:241976A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84619-5-PBS in a different storage buffer formulation.

  • FC experiment of HT-1080 using 84619-5-RR (same clone as 84619-5-PBS)

    FC experiment of HT-1080 using 84619-5-RR (same clone as 84619-5-PBS)

    1x10^6 HT-1080 cells were intracellularly stained with 0.25 ug FADD Recombinant antibody (84619-5-RR, Clone:241976A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84619-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84619-5-RR (same clone as 84619-5-PBS)

    IF Staining of HeLa using 84619-5-RR (same clone as 84619-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FADD antibody (84619-5-RR, Clone: 241976A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84619-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84619-5-RR

    Affinity and Kinetic Characterization of 84619-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84619-5-RR against Human FADD were performed. The affinity constant is 19.8 nM.

FADD Recombinant antibody, PBS Only

FADD Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84619-5-PBS

产品说明书

CloneNo. 241976A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

MORT1, Growth-inhibiting gene 3 protein, FAS-associating death domain-containing protein, FAS-associated death domain protein, 241976A11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FADD Ready-To-Use IHC Kit. FADD primary antibody included.

产品信息

84619-5-PBS targets FADD in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 FADD fusion protein Ag6701 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fas (TNFRSF6)-associated via death domain
别名 MORT1, Growth-inhibiting gene 3 protein, FAS-associating death domain-containing protein, FAS-associated death domain protein, 241976A11
计算分子量 23 kDa
观测分子量29 kDa
GenBank蛋白编号BC000334
基因名称 FADD
Gene ID (NCBI) 8772
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13158
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)