• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of mouse peritoneal macrophages using CL750-98236

    FC experiment of mouse peritoneal macrophages using CL750-98236

    1x10^6 mouse peritoneal macrophages were surface stained with FITC Plus Anti-Mouse CD11b, and 0.25 ug CoraLite® Plus 750 Anti-Mouse F4/80 Rabbit RecAb (CL750-98236, Clone: 241959G4) or 0.25 ug CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

  • FC experiment of mouse peritoneal macrophages using CL750-98236

    FC experiment of mouse peritoneal macrophages using CL750-98236

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite® Plus 750 Anti-Mouse F4/80 Rabbit RecAb (CL750-98236, Clone: 241959G4) (red) or 0.25 ug CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

CoraLite® Plus 750 Anti-Mouse F4/80 Rabbit Recombinant Antibody

F4/80 Uni-rAbTM Recombinant Antibody for FC
Cat No. CL750-98236

产品说明书

CloneNo. 241959G4

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inmouse peritoneal macrophages
Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98236-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-98236 targets F4/80 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 EGF-like module containing, mucin-like, hormone receptor-like sequence 1
别名 Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1
计算分子量102 kDa
GenBank蛋白编号NM_010130.4
基因名称 F4/80
Gene ID (NCBI) 13733
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ61549
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

实验方案

Product Specific Protocols
FC protocol for CL Plus 750 F4/80 antibody CL750-98236Download protocol
Standard Protocols
Click here to view our Standard Protocols