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Mouse F4/80 Recombinant antibody, PBS Only

F4/80 Recombinant Antibody for Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2P18

Cat No : 81668-1-PBS

Print datasheet

Synonyms

DD7A5 7, EGF TM7, Emr1, F4 80, F4/80, Gpf480, Ly71, TM7LN3

验证数据展示

  • IHC staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

    IHC staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Mouse F4/80 antibody included
  • IHC staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

    IHC staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Mouse F4/80 antibody included
  • IF Staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

    IF Staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

    IF Staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.

产品信息

81668-1-PBS targets F4/80 in IF, IHC, Indirect ELISA applications and shows reactivity with Mouse samples.

Tested Applications Indirect ELISA,IHC,IF
Tested Reactivity Mouse
Immunogen F4/80 fusion protein Ag28252 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name EGF-like module containing, mucin-like, hormone receptor-like sequence 1
Synonyms DD7A5 7, EGF TM7, Emr1, F4 80, F4/80, Gpf480, Ly71, TM7LN3
Calculated Molecular Weight 102 kDa
GenBank Accession NumberNM_010130
Gene Symbol Emr1
Gene ID (NCBI) 13733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ61549
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.