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验证数据展示

  • WB analysis using 84449-1-RR

    WB analysis using 84449-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84449-1-RR (XPO4 antibody) at dilution of 1:8500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 84449-1-RR

    WB analysis of HEK-293 using 84449-1-RR

    WB result of Exportin 4 antibody (84449-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Exportin 4 transfected HEK-293 cells.

  • IF Staining of K-562 using 84449-1-RR

    IF Staining of K-562 using 84449-1-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Exportin 4 antibody (84449-1-RR, Clone: 241443A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 84449-1-RR

    FC experiment of HeLa using 84449-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Exportin 4 Recombinant antibody (84449-1-RR, Clone:241443A5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84449-1-RR

    Affinity and Kinetic Characterization of 84449-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84449-1-RR against Human Exportin 4 were performed. The affinity constant is 0.512 nM.

  • Featured Product
  • KD/KO验证

Exportin 4 Recombinant monoclonal antibody

Exportin 4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84449-1-RR

产品说明书

CloneNo. 241443A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

XPO4, 241443A5, Exp4, Exportin-4, KIAA1721

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, SW480 cells, RAW 264.7 cells
Positive IF/ICC detected inK-562 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:17000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84449-1-RR targets Exportin 4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21845

Product name: Recombinant human XPO4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 776-1124 aa of BC113571

Sequence: QEITATLEALCGIAEATQIDNVAILFNFLMDFLTNCIGLMEVYKNTPETVNLIIEVFVEVAHKQICYLGESKAMNLYEACLTLLQVYSKNNLGRQRIDVTAEEEQYQDLLLIMELLTNLLSKEFIDFSDTDEVFRGHEPGQAANRSVSAADVVLYGVNLILPLMSQDLLKFPTLCNQYYKLITFICEIFPEKIPQLPEDLFKSLMYSLELGMTSMSSEVCQLCLEALTPLAEQCAKAQETDSPLFLATRHFLKLVFDMLVLQKHNTEMTTAAGEAFYTLVCLHQAEYSELVETLLSSQQDPVIYQRLADAFNKLTASSTPPTLDRKQKMAFLKSLEEFMANVGGLLCVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 exportin 4
别名 XPO4, 241443A5, Exp4, Exportin-4, KIAA1721
计算分子量 1151 aa, 130 kDa
观测分子量130 kDa
GenBank蛋白编号BC113571
基因名称 Exportin 4/XPO4
Gene ID (NCBI) 64328
RRIDAB_3671975
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9C0E2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

exportin 4 (XPO4) maps to chromosome 13q and belongs to the importin β family which acts as a bidirectional nuclear transport receptor. Decreased XPO4 expression has been shown to associate with hepatocellular carcinoma. A genome-wide copy number variation (CNV) association study in a small cohort of Malaysian subjects identified a CNV in XPO4 to be correlated with the progression of metabolic (dysfunction) associated fatty liver disease (MAFLD). (PMID: 34388518)

实验方案

Product Specific Protocols
IF protocol for Exportin 4 antibody 84449-1-RRDownload protocol
WB protocol for Exportin 4 antibody 84449-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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