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验证数据展示

  • WB analysis using 12999-1-AP (same clone as 12999-1-PBS)

    WB analysis using 12999-1-AP (same clone as 12999-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12999-1-AP (Ephrin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12999-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12999-1-AP (same clone as 12999-1-PBS)

    IHC staining of human kidney using 12999-1-AP (same clone as 12999-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12999-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12999-1-AP (same clone as 12999-1-PBS)

    IHC staining of human kidney using 12999-1-AP (same clone as 12999-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12999-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Ephrin B1 Polyclonal antibody, PBS Only

Ephrin B1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12999-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

EFL3, EFNB1, EPLG2, LERK2, EFL-3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12999-1-PBS targets Ephrin B1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3669

Product name: Recombinant human EFNB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 32-239 aa of BC016649

Sequence: LEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAGRPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNRPEQEIRFTIKFQEFSPNYMGLEFKKHHDYYITSTSNGSLEGLENREGGVCRTRTMKIIMKVGQDPNAVTPEQLTTSRPSKEADNTVKMATQAPGSRGSLGDSDGKHETVNQEEKSGPGASGGSSGDPDGFFNSKVA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ephrin-B1
别名 EFL3, EFNB1, EPLG2, LERK2, EFL-3
计算分子量 346 aa, 38 kDa
观测分子量38 kDa
GenBank蛋白编号BC016649
基因名称 Ephrin B1
Gene ID (NCBI) 1947
RRIDAB_10644156
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP98172
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EFNB1 (Ephrin B1) is a member of the ephrin family, which are membrane-bound guidance cues that interact with Eph receptors, a family of receptor tyrosine kinases. EFNB1 is X-inactivated, and it has been proposed that in heterozygous females, patchy loss of ephrin B1 disturbs tissue boundary formation at the developing coronal suture (PMID: 34824583, 18627045). EFNB1 mainly plays a central part in cell adhesion, angiogenesis, and development of the nervous system (PMID: 30326247).

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