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Ephrin B1 Polyclonal antibody

Ephrin B1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12999-1-AP

Print datasheet

Synonyms

CFND, CFNS, EFL 3, EFL3, EFNB1, Elk L, ELK ligand, ephrin B1, EPLG2, LERK 2, LERK2

验证数据展示

  • WB analysis using 12999-1-AP

    WB analysis using 12999-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12999-1-AP (Ephrin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 12999-1-AP

    IHC staining of human kidney using 12999-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 12999-1-AP

    IHC staining of human kidney using 12999-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, rat kidney tissue
Positive IHC detected inhuman kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12999-1-AP targets Ephrin B1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Ephrin B1 fusion protein Ag3669 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ephrin-B1
Synonyms CFND, CFNS, EFL 3, EFL3, EFNB1, Elk L, ELK ligand, ephrin B1, EPLG2, LERK 2, LERK2
Calculated Molecular Weight 346 aa, 38 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC016649
Gene Symbol EFNB1
Gene ID (NCBI) 1947
RRIDAB_10644156
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP98172
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Ephrin B1 antibody 12999-1-APDownload protocol
IHC protocol for Ephrin B1 antibody 12999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Signal Transduct Target Ther

Aberrant epithelial cell interaction promotes esophageal squamous-cell carcinoma development and progression

Authors - Liping Chen
  • KD Validated
View Article