验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88021-1-RR targets Epha4 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Eg8776 Product name: Recombinant mouse Epha4 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 20-547 aa of NM_007936.3 Sequence: VTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEASQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRESQFGKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEEQNGECQACKIGYYKALSTDASCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTRVSITDLLAHTNYTFEIWAVNGVSKYNPSPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANST 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Eph receptor A4 |
| 别名 | |
| 计算分子量 | 110 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | NM_007936.3 |
| 基因名称 | Epha4 |
| Gene ID (NCBI) | 13838 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q03137-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EPH receptor A4 (EPHA4) is a member of the Eph receptor family of receptor tyrosine kinases (RTKs) that interacts with both ephrin-A and ephrin-B ligands, making it one of the most versatile Eph receptors. In mice, Epha4 is highly expressed in the central nervous system, particularly during development, where it regulates axon guidance, neuronal pathfinding, synaptic plasticity, and neural circuit formation. EPHA4 mediates contact-dependent bidirectional signaling, often inducing cell repulsion and boundary formation, which is critical for proper tissue patterning. Beyond development, EPHA4 continues to function in the adult CNS, contributing to synaptic remodeling and neuronal regeneration responses.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Epha4 antibody 88021-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
