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验证数据展示

  • WB analysis using 84490-2-RR (same clone as 84490-2-PBS)

    WB analysis using 84490-2-RR (same clone as 84490-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84490-2-RR (EpCAM/TROP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 84490-2-RR (same clone as 84490-2-PBS)

    IHC staining of mouse colon using 84490-2-RR (same clone as 84490-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 84490-2-RR (same clone as 84490-2-PBS)

    IHC staining of mouse colon using 84490-2-RR (same clone as 84490-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01344-1

    Sandwich ELISA standard curve of MP01344-1

    Sandwich ELISA standard curve of MP01344-1, Mouse EPCAM/CD326 Recombinant Matched Antibody Pair - PBS only. 84490-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1883. 84490-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

  • Sample test of MP01344-1

    Sample test of MP01344-1

    The mean EPCAM/CD326 concentration was determined to be 76,517.6 pg/mL in mouse kidney tissue extract based on a 3.1 mg/mL extract load.

  • IF Staining of mouse colon using 84490-2-RR (same clone as 84490-2-PBS)

    IF Staining of mouse colon using 84490-2-RR (same clone as 84490-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using EPCAM/CD326 antibody (84490-2-RR, Clone: 241851E11 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 84490-2-RR (same clone as 84490-2-PBS)

    IF Staining of mouse small intestine using 84490-2-RR (same clone as 84490-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using EPCAM/CD326 antibody (84490-2-RR, Clone: 241851E11 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84490-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01344-1

    Cytometric bead array standard curve of MP01344-1

    Cytometric bead array standard curve of MP01344-1, MOUSE EPCAM/CD326 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84490-2-PBS. Detection antibody: 84490-3-PBS. Standard: Eg1883. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP01344-2

    Cytometric bead array standard curve of MP01344-2

    Cytometric bead array standard curve of MP01344-2, MOUSE EPCAM/CD326 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84490-2-PBS. Detection antibody: 84490-1-PBS. Standard: Eg1883. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 84490-2-RR

    Affinity and Kinetic Characterization of 84490-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84490-2-RR against Mouse EPCAM/CD326 were performed. The affinity constant is 0.779 nM.

Mouse EPCAM/CD326 Recombinant antibody, PBS Only (Capture)

EPCAM/CD326 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84490-2-PBS

产品说明书

CloneNo. 241851E11

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

241851E11, CD326, EGP314, Epcam, Ep-CAM

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84490-2-PBS targets EPCAM/CD326 as part of a matched antibody pair:

MP01344-1: 84490-2-PBS capture and 84490-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01344-2: 84490-2-PBS capture and 84490-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 mouse
免疫原 EPCAM/CD326 fusion protein Eg1883 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 epithelial cell adhesion molecule
别名 241851E11, CD326, EGP314, Epcam, Ep-CAM
计算分子量35 kDa
观测分子量35 kDa, 40 kDa
GenBank蛋白编号NM_008532.2
基因名称 Epcam
Gene ID (NCBI) 17075
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99JW5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)