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验证数据展示

  • WB analysis using 84490-2-RR

    WB analysis using 84490-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84490-2-RR (EpCAM/TROP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 84490-2-RR

    IHC staining of mouse colon using 84490-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 84490-2-RR

    IHC staining of mouse colon using 84490-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84490-2-RR

    Affinity and Kinetic Characterization of 84490-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84490-2-RR against Mouse EPCAM/CD326 were performed. The affinity constant is 0.779 nM.

EPCAM/CD326 Recombinant antibody

EPCAM/CD326 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84490-2-RR

产品说明书

CloneNo. 241851E11

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Epithelial cell adhesion molecule, Ep-CAM, Epcam, EGP314, CD326

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse colon tissue, mouse small intestine tissue
Positive IHC detected inmouse colon tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84490-2-RR targets EPCAM/CD326 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 epithelial cell adhesion molecule
别名 Epithelial cell adhesion molecule, Ep-CAM, Epcam, EGP314, CD326
计算分子量35 kDa
观测分子量35 kDa, 40 kDa
GenBank蛋白编号NM_008532.2
基因名称 Epcam
Gene ID (NCBI) 17075
RRIDAB_3672005
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99JW5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

实验方案

Product Specific Protocols
WB protocol for EPCAM/CD326 antibody 84490-2-RRDownload protocol
IHC protocol for EPCAM/CD326 antibody 84490-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols