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验证数据展示

  • WB analysis using 84807-4-RR

    WB analysis using 84807-4-RR

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 84807-4-RR (Endoglin/CD105 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of bEnd.3 using 84807-4-RR

    IF Staining of bEnd.3 using 84807-4-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using Endoglin/CD105 antibody (84807-4-RR, Clone: 242305G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse kidney using 84807-4-RR

    IHC staining of mouse kidney using 84807-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84807-4-RR (Endoglin/CD105 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 84807-4-RR

    IHC staining of mouse kidney using 84807-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84807-4-RR (Endoglin/CD105 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84807-4-RR

    Affinity and Kinetic Characterization of 84807-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84807-4-RR against Mouse Endoglin/CD105 were performed. The affinity constant is 0.888 nM.

Endoglin/CD105 Recombinant antibody

Endoglin/CD105 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84807-4-RR

产品说明书

CloneNo. 242305G3

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CD105, 242305G3, S endoglin, Eng, endoglin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inbEnd.3 cells, mouse lung tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inbEnd.3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84807-4-RR targets Endoglin/CD105 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 endoglin
别名 CD105, 242305G3, S endoglin, Eng, endoglin
计算分子量70kDa
观测分子量85-90 kDa
GenBank蛋白编号NM_007932.2
基因名称 CD105
Gene ID (NCBI) 13805
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ63961
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodeling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 84807-4-RRDownload protocol
IHC protocol for Endoglin/CD105 antibody 84807-4-RRDownload protocol
IF protocol for Endoglin/CD105 antibody 84807-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols