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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated EZH2 Polyclonal antibody

EZH2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-21800

Print datasheet

Synonyms

Enhancer of zeste homolog 2, ENX 1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6

验证数据展示

  • FC experiment of HepG2 using CL647-21800

    FC experiment of HepG2 using CL647-21800

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human EZH2 (CL647-21800) (red), or 0.2 ug 0.2 ug CL647 rabbit Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHepG2 cells

This antibody is not recommended for IHC.

For other applications, we recommend the unconjugated version of this antibody, 21800-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL647-21800 is EZH2 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen EZH2 fusion protein Ag16789 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enhancer of zeste homolog 2 (Drosophila)
Synonyms Enhancer of zeste homolog 2, ENX 1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6
Calculated Molecular Weight 751 aa, 86 kDa
Observed Molecular Weight 90-102 kDa
GenBank Accession NumberBC010858
Gene Symbol EZH2
Gene ID (NCBI) 2146
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15910
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 EZH2 antibody CL647-21800Download protocol
Standard Protocols
Click here to view our Standard Protocols