×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of HeLa using 21196-1-AP (same clone as 21196-1-PBS)

    WB analysis of HeLa using 21196-1-AP (same clone as 21196-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • IP experiment of L02 using 21196-1-AP (same clone as 21196-1-PBS)

    IP experiment of L02 using 21196-1-AP (same clone as 21196-1-PBS)

    IP result of anti-EYA3 (IP:21196-1-AP, 4ug; Detection:21196-1-AP 1:1000) with L02 cells lysate 1800ug. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

    IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

    IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 21196-1-AP (same clone as 21196-1-PBS)

    FC experiment of A431 using 21196-1-AP (same clone as 21196-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug EYA3 Polyclonal antibody (21196-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 21196-1-AP (same clone as 21196-1-PBS)

    IF Staining of HeLa using 21196-1-AP (same clone as 21196-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA3 antibody (21196-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EYA3 Polyclonal antibody, PBS Only

EYA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21196-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EC:3.1.3.48

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

21196-1-PBS targets EYA3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15544

Product name: Recombinant human EYA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC029500

Sequence: MEEEQDLPEQPVKKAKMQESGEQTISQVSNPDVSDQKPETSSLASNLPMSEEIMTCTDYIPRSSNDYTSQMYSAKPYAHILSVPVSETAYPGQTQYQTLQQTQPYAVYPQATQTYGLPPFGALWPGMKPESGLIQTPSPSQHSVLTCTTGLTTSQPSPAHYSYPIQASSTNASLISTSSTIANIPAAAVASISNQDYPTYTILGQNQYQACYPSSSFGVTGQTNSDAESTTLAATTYQSEKPSVMAPAPAAQRLSSGDPSTSPSLSQTTPSKDTDDQSRKNMTSKNRGKRKADATSSQDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eyes absent homolog 3 (Drosophila)
别名 EC:3.1.3.48
计算分子量 573 aa, 63 kDa
观测分子量 63-66 kDa
GenBank蛋白编号BC029500
基因名称 EYA3
Gene ID (NCBI) 2140
RRIDAB_10732957
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99504
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EYA3(Eyes absent homolog 3) belongs to the HAD-like hydrolase superfamily.Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis.EYA3, like EYA1, is expressed in the ninth week of human development and may also underlie developmental defects(PMID:9020840).It has 2 isoforms produced by alternative splicing.This antibody is specific to EYA3.

||
New chat

Able™

正在加载，请稍候...