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验证数据展示

  • WB analysis using 11314-1-AP (same clone as 11314-1-PBS)

    WB analysis using 11314-1-AP (same clone as 11314-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11314-1-AP (EYA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11314-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 11314-1-AP (same clone as 11314-1-PBS)

    IHC staining of human prostate cancer using 11314-1-AP (same clone as 11314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11314-1-AP (EYA2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11314-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EYA2 antibody included
  • IF Staining of HeLa using 11314-1-AP (same clone as 11314-1-PBS)

    IF Staining of HeLa using 11314-1-AP (same clone as 11314-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA2 antibody (11314-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11314-1-PBS in a different storage buffer formulation.

EYA2 Polyclonal antibody, PBS Only

EYA2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11314-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:3.1.3.48, EAB1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EYA2 Ready-To-Use IHC Kit. EYA2 primary antibody included.

产品信息

11314-1-PBS targets EYA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1860

Product name: Recombinant human EYA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 241-583 aa of BC013882

Sequence: LSYGSSFSTSPTGQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGKESCFERIMQRFGRKAVYVVIGDGVEEEQGAKKHNMPFWRISCHADLEALRHALELEYL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eyes absent homolog 2 (Drosophila)
别名 EC:3.1.3.48, EAB1
计算分子量 64 kDa
观测分子量56 kDa and 70 kDa
GenBank蛋白编号BC013882
基因名称 EYA2
Gene ID (NCBI) 2139
RRIDAB_10733651
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00167
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EYA2, also named EAB1, belongs to the HAD-like hydrolase superfamily and EYA family. It coactivates SIX1. The Six1-EYA2 interaction may inhibit breast cancer progression. EYA2 is required for Six1-activated TGF-beta signaling.(PMID:21706047) It may be involved in the development of the eye. This protein has 3 isoforms produced by alternative splicing with the molecular weight of 59 kDa, 57 kDa, and 50 kDa. It can be detected two bands of 70 kDa and 56 kDa in the neuroblastoma cells by western blot(PMID:12039049).

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