验证数据展示
经过测试的应用
| Positive IP detected in | HEK-293T cells, mouse pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 2 publications below |
产品信息
10588-1-AP targets EXTL3 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | EXTL3 fusion protein Ag0936 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exostoses (multiple)-like 3 |
| 别名 | EC:2.4.1.223, Exostosin-like 3, EXTL1L, EXTR1, EXT-related protein 1 |
| 计算分子量 | 105 kDa |
| 观测分子量 | 105 kDa |
| GenBank蛋白编号 | BC006363 |
| 基因名称 | EXTL3 |
| Gene ID (NCBI) | 2137 |
| RRID | AB_2262577 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43909 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Exostosin like glycosyltransferase 3 (EXTL3), also known as RPR, is located at chromosome 8p21.1 and composed of 919 amino acids, making it the longest member of the EXT family (PMID: 35676258). EXTL3 encodes the glycosyltransferases responsible for the biosynthesis of the backbone structure of heparan sulfate (HS) (PMID: 24128412). EXTL3 has been identified as a receptor molecule for regenerating islet-derived (REG) protein ligands, which have been shown to stimulate islet β-cell growth. Mutations in EXTL3 could lead to skeletal dysplasia, developmental delay, and severe immune deficiency (PMID: 28148688). The predicted molecular weight is 105 kDa, but we detected the band at 65 kDa perhaps due to alternative splicing (PMID: 14618397).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for EXTL3 antibody 10588-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|