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验证数据展示

  • WB analysis using 67636-1-Ig (same clone as 67636-1-PBS)

    WB analysis using 67636-1-Ig (same clone as 67636-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67636-1-Ig (EXOSC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

    WB analysis of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

    WB result of EXOSC9 antibody (67636-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells. This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67636-1-Ig (same clone as 67636-1-PBS)

    IHC staining of human breast cancer using 67636-1-Ig (same clone as 67636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EXOSC9 antibody included
  • IF Staining of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

    IF Staining of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC9 antibody (67636-1-Ig, Clone: 3F4G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51449-1

    Cytometric bead array sample test of MP51449-1

    Cytometric bead array sample test of MP51449-1, EXOSC9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67636-1-PBS. Detection antibody: 67636-2-PBS.

  • Cytometric bead array standard curve of MP51449-1

    Cytometric bead array standard curve of MP51449-1

    Cytometric bead array standard curve of MP51449-1, EXOSC9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67636-1-PBS. Detection antibody: 67636-2-PBS. Standard:Ag19783. Range: 3.125-400 ng/mL.

  • Featured Product
  • KD/KO验证

EXOSC9 Monoclonal antibody, PBS Only (Capture)

EXOSC9 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67636-1-PBS

产品说明书

CloneNo. 3F4G1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

3F4G1, Exosome complex component RRP45, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, PM/Scl-75, PMSCL1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EXOSC9 Ready-To-Use IHC Kit. EXOSC9 primary antibody included.

产品信息

67636-1-PBS targets EXOSC9 as part of a matched antibody pair:

MP51449-1: 67636-1-PBS capture and 67636-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19783

Product name: Recombinant human EXOSC9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC142978

Sequence: MKETPLSNCERRFLLRAIEEKKRLDGRQTYDYRNIRISFGTDYGCCIVELGKTRVLGQVSCELVSPKLNRATEGILFFNLELSQMAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGAYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLE

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 exosome component 9
别名 3F4G1, Exosome complex component RRP45, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, PM/Scl-75, PMSCL1
计算分子量 456 aa, 51 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC142978
基因名称 EXOSC9
Gene ID (NCBI) 5393
RRIDAB_2882837
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ06265
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

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