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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated EXOSC3 Polyclonal antibody

EXOSC3 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-15062

Print datasheet

Synonyms

bA3J10.7, CGI 102, EXOSC3, exosome component 3, hRrp 40, hRrp40p, p10, RP11 3J10.8, RRP40, Rrp40p

验证数据展示

  • IF Staining of PC-3 using CL488-15062

    IF Staining of PC-3 using CL488-15062

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite® Plus 488 EXOSC3 antibody (CL488-15062) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy EXOSC3 Ready-To-Use IHC Kit. EXOSC3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15062-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15062 targets EXOSC3 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen EXOSC3 fusion protein Ag7065 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name exosome component 3
Synonyms bA3J10.7, CGI 102, EXOSC3, exosome component 3, hRrp 40, hRrp40p, p10, RP11 3J10.8, RRP40, Rrp40p
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC002437
Gene Symbol EXOSC3
Gene ID (NCBI) 51010
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQT5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA exosomes are multi-subunit complexes conserved throughout evolution, and they are emerging as the major cellular machinery for processing, surveillance and turnover of a diverse spectrum of coding and noncoding RNA substrates essential for viability[PMID:22544365]. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts [PMID:11782436]. EXOSC3 is a non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of Rnase PH-domain subunits through contacts with EXOSC9 and EXOSC5 [PMID:21255825].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EXOSC3 antibody CL488-15062Download protocol
Standard Protocols
Click here to view our Standard Protocols