验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, mouse brain tissue, mouse pancreas tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20138-1-AP targets EXD2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | EXD2 fusion protein Ag14090 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exonuclease 3'-5' domain containing 2 |
| 别名 | 3'-5' exoribonuclease EXD2, C14orf114, EC:3.1.11.1, EC:3.1.13.-, EXDL2 |
| 计算分子量 | 70 kD aa, 56 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC001962 |
| 基因名称 | EXD2 |
| Gene ID (NCBI) | 55218 |
| RRID | AB_10665419 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NVH0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EXD2 (Exonuclease 3'-5' Domain Containing 2), is a protein with exonuclease activity of 3'-5' nucleic acid, which is involved in many biological processes in cells, including DNA repair, RNA processing and mitochondrial translation. EXD2 protein is mainly located in mitochondrial outer membrane, intermediate fiber cytoskeleton and DNA damage site. This protein may be a potential drug target in some cancers. For example, in ALT (telomere selective elongation) dependent cancer, the co-elimination of EXD2 with BLM, DNA2 or POLD3 may mediate synthetic lethality. In addition, EXD2 inhibitors may exert anticancer effects by inhibiting mitochondrial translation and metabolic reprogramming. It has two isoforms with molecular weights of 56 kDa and 70 kDa respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EXD2 antibody 20138-1-AP | Download protocol |
| IHC protocol for EXD2 antibody 20138-1-AP | Download protocol |
| IF protocol for EXD2 antibody 20138-1-AP | Download protocol |
| IP protocol for EXD2 antibody 20138-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Multiple DSB Resection Activities Redundantly Promote Alternative End Joining-Mediated Class Switch Recombination.
| ||
Andrology Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes |
