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验证数据展示

  • IF Staining of U2OS using 84738-2-RR (same clone as 84738-2-PBS)

    IF Staining of U2OS using 84738-2-RR (same clone as 84738-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EVI5 antibody (84738-2-RR, Clone: 241918D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84738-2-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84738-2-RR (same clone as 84738-2-PBS)

    FC experiment of U2OS using 84738-2-RR (same clone as 84738-2-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug EVI5 Recombinant antibody (84738-2-RR, Clone:241918D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84738-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 84738-2-RR (same clone as 84738-2-PBS)

    IHC staining of mouse stomach using 84738-2-RR (same clone as 84738-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84738-2-RR (EVI5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84738-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84738-2-RR

    Affinity and Kinetic Characterization of 84738-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84738-2-RR against Human EVI5 were performed. The affinity constant is 1.11 nM.

EVI5 Recombinant monoclonal antibody, PBS Only

EVI5 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84738-2-PBS

产品说明书

CloneNo. 241918D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

241918D11, NB4S

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84738-2-PBS targets EVI5 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26739

Product name: Recombinant human EVI5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-80 aa of BC156793

Sequence: MVTNKMTAAFRNPSGKQVATDKVAEKLSSTLSWVKNTVSHTVSQMASQVASPSTSLHTTSSSTTLSTPALSPSSPSQLSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ecotropic viral integration site 5
别名 241918D11, NB4S
计算分子量 810 aa, 93 kDa
GenBank蛋白编号BC156793
基因名称 EVI5
Gene ID (NCBI) 7813
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO60447
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EVI5 functions as a regulator of cell cycle progression by stabilizing the FBXO5 protein and promoting cyclin-A accumulation during interphase. It may play a role in cytokinesis (PMID: 16439210).

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