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验证数据展示

  • WB analysis using 55367-1-AP

    WB analysis using 55367-1-AP

    Mouse lung tissue were subjected to SDS PAGE followed by western blot with 55367-1-AP (EVC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 55367-1-AP

    IHC staining of human placenta using 55367-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55367-1-AP (EVC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EVC2 Polyclonal antibody

EVC2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55367-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Mouse, Human

应用

WB, IHC, ELISA

别名

Ellis van Creveld syndrome 2, EVC2, LBN, Limbin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55367-1-AP targets EVC2 in WB, IHC, ELISA applications and shows reactivity with Mouse, Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Mouse, Human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ellis van Creveld syndrome 2
别名 Ellis van Creveld syndrome 2, EVC2, LBN, Limbin
计算分子量 148 kDa
观测分子量140 kDa
GenBank蛋白编号NM_147127
基因名称 EVC2
Gene ID (NCBI) 132884
RRIDAB_3086445
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UK5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EVC2 (also known as LIMBIN) is close proximity in divergent orientation to EVC and mutations in both human genes lead to the chondrodysplasia Ellis-van Creveld (EvC) syndrome. Typical symptoms of EvC include shorter limbs and ribs, postaxial polydactyly, dysplastic nails, and cardiovascular defects (PMID: 28081373). Additionally, EvC syndrome has been characterized as a ciliopathy because the EVC and EVC2 proteins are intracellularly localized at the bottom of the primary cilium, where they form a heterotrimeric protein complex with SMO and transduce Hedgehog(Hh) signaling (PMID: 30410447). EVC2 is expressed in the heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. EVC2 has 3 isoforms with the molecular mass of 140-148 kDa.

实验方案

Product Specific Protocols
WB protocol for EVC2 antibody 55367-1-APDownload protocol
IHC protocol for EVC2 antibody 55367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols