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验证数据展示

  • WB analysis of BGC-823 using 27786-1-AP

    WB analysis of BGC-823 using 27786-1-AP

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 27786-1-AP (ETHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27786-1-AP

    IHC staining of human liver using 27786-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ETHE1 antibody included
  • IHC staining of human liver using 27786-1-AP

    IHC staining of human liver using 27786-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ETHE1 antibody included

ETHE1 Polyclonal antibody

ETHE1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27786-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse and More (1)

应用

WB, IHC, ELISA

别名

ETHE1, ethylmalonic encephalopathy 1, HSCO, Protein ETHE1, mitochondrial, YF13H12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBGC-823 cells, HeLa cells, mouse liver tissue
Positive IHC detected inhuman liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ETHE1 Ready-To-Use IHC Kit. ETHE1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27786-1-AP targets ETHE1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, mouse
文献引用反应性human
免疫原 ETHE1 fusion protein Ag26597 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ethylmalonic encephalopathy 1
别名 ETHE1, ethylmalonic encephalopathy 1, HSCO, Protein ETHE1, mitochondrial, YF13H12
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC008250
基因名称 ETHE1
Gene ID (NCBI) 23474
RRIDAB_2880971
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95571
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ETHE1, a gene encoding a b-lactamase-like, iron-coordinating metalloprotein, is mutated in ethylmalonic encephalopathy.In humans, ETHE1 gene is proved to encode the SDO in mitochondria and its mutations lead to a fatal autosomal recessive mitochondrial disease: ethylmalonic encephalopathy.

实验方案

Product Specific Protocols
WB protocol for ETHE1 antibody 27786-1-APDownload protocol
IHC protocol for ETHE1 antibody 27786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

Phosphorylated FOXQ1, a novel substrate of JNK1, inhibits sorafenib-induced ferroptosis by activating ETHE1 in hepatocellular carcinoma

Authors - Yiwei Liu
View Article