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验证数据展示

  • WB analysis of BGC-823 using 27786-1-AP

    WB analysis of BGC-823 using 27786-1-AP

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 27786-1-AP (ETHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27786-1-AP

    IHC staining of human liver using 27786-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ETHE1 antibody included
  • IHC staining of human liver using 27786-1-AP

    IHC staining of human liver using 27786-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ETHE1 antibody included

ETHE1 Polyclonal antibody

ETHE1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27786-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse and More (1)

应用

WB, IHC, ELISA

别名

ETHE1, ethylmalonic encephalopathy 1, HSCO, Protein ETHE1, mitochondrial, YF13H12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBGC-823 cells, HeLa cells, mouse liver tissue
Positive IHC detected inhuman liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ETHE1 Ready-To-Use IHC Kit. ETHE1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27786-1-AP targets ETHE1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, mouse
文献引用反应性human
免疫原

CatNo: Ag26597

Product name: Recombinant human ETHE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 8-132 aa of BC008250

Sequence: VARRQLSQRGGSGAPILLRQMFEPVSCTFTYLLGDRESREAVLIDPVLETAPRDAQLIKELGLRLLYAVNTHCHADHITGSGLLRSLLPGCQSVISRLSGAQADLHIEDGDSIRFGRFALETRAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ethylmalonic encephalopathy 1
别名 ETHE1, ethylmalonic encephalopathy 1, HSCO, Protein ETHE1, mitochondrial, YF13H12
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC008250
基因名称 ETHE1
Gene ID (NCBI) 23474
RRIDAB_2880971
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95571
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ETHE1, a gene encoding a b-lactamase-like, iron-coordinating metalloprotein, is mutated in ethylmalonic encephalopathy.In humans, ETHE1 gene is proved to encode the SDO in mitochondria and its mutations lead to a fatal autosomal recessive mitochondrial disease: ethylmalonic encephalopathy.

实验方案

Product Specific Protocols
WB protocol for ETHE1 antibody 27786-1-APDownload protocol
IHC protocol for ETHE1 antibody 27786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

Phosphorylated FOXQ1, a novel substrate of JNK1, inhibits sorafenib-induced ferroptosis by activating ETHE1 in hepatocellular carcinoma

Authors - Yiwei Liu
View Article