• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66818-1-Ig (same clone as 66818-1-PBS)

    WB analysis using 66818-1-Ig (same clone as 66818-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

  • WB analysis using 66818-1-Ig (same clone as 66818-1-PBS)

    WB analysis using 66818-1-Ig (same clone as 66818-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66818-1-Ig (ESRRB antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66818-1-Ig (same clone as 66818-1-PBS)

    IHC staining of human prostate cancer using 66818-1-Ig (same clone as 66818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66818-1-Ig (ESRRB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ESRRB antibody included
  • IF Staining of Caco-2 using 66818-1-Ig (same clone as 66818-1-PBS)

    IF Staining of Caco-2 using 66818-1-Ig (same clone as 66818-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ESRRB antibody (66818-1-Ig, Clone: 3E10C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), Alpha Tubulin antibody (80762-1-RR, Clone: 1G7, red). This data was developed using the same antibody clone with 66818-1-PBS in a different storage buffer formulation.

ESRRB Monoclonal antibody, PBS Only

ESRRB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66818-1-PBS

产品说明书

CloneNo. 3E10C1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ERR-beta, ERRbeta, ERRB2, ERRb, ERR beta-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy ESRRB Ready-To-Use IHC Kit. ESRRB primary antibody included.

产品信息

66818-1-PBS targets ESRRB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ESRRB fusion protein Ag18626 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 estrogen-related receptor beta
别名 ERR-beta, ERRbeta, ERRB2, ERRb, ERR beta-2
计算分子量 508 aa, 56 kDa
观测分子量 47 kDa
GenBank蛋白编号BC131517
基因名称 ESRRB
Gene ID (NCBI) 2103
RRIDAB_2882161
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95718
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.