验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, mouse lung tissue, MCF-7 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human lung tissue, mouse colon tissue, human bowen disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21045-1-AP targets ESRP1 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag15386 Product name: Recombinant human ESRP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-226 aa of BC099916 Sequence: EKELILLFWKVVDLANKKVGQLHEVLVRPDQLELTEDCKEETKIDVESLSSASQLDQALRQFNQSVSNELNIGVGTSFCLCTDGQLHVRQILHPEASKKNVLLPECFYSFFDLRKEFKKCCPGSPDIDKLDVATMTEYLNFEKSSSVSRYGASQVEDMGNIILAMISEPYNHRFSDPERVNYKFESGTCSKMELIDDNTV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | epithelial splicing regulatory protein 1 |
| 别名 | RNA-binding protein 35A, RNA-binding motif protein 35A, RNA binding protein 35A, RNA binding motif protein 35A, RBM35A |
| 计算分子量 | 681 aa, 76 kDa |
| 观测分子量 | 76 kDa |
| GenBank蛋白编号 | BC099916 |
| 基因名称 | ESRP1 |
| Gene ID (NCBI) | 54845 |
| RRID | AB_2878801 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6NXG1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ESRP1, also named as Epithelial splicing regulatory protein 1, is a 681 amino acid protein, which belongs to the ESRP family. ESRP1 as a mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. ESRP1 specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2 and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). ESRP1 exists various isoforms and molecular weight of each isoform is 76 kDa, 67 kDa, and 73 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ESRP1 antibody 21045-1-AP | Download protocol |
| IP protocol for ESRP1 antibody 21045-1-AP | Download protocol |
| WB protocol for ESRP1 antibody 21045-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res ESRP1 controls biogenesis and function of a large abundant multiexon circRNA
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Mol Ther circCAMSAP1 Promotes Tumor Growth in Colorectal Cancer via the miR-328-5p/E2F1 Axis.
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Cell Death Differ Splicing factor derived circular RNA circUHRF1 accelerates oral squamous cell carcinoma tumorigenesis via feedback loop. | ||
Oncogene Androgen receptor signaling regulates the transcriptome of prostate cancer cells by modulating global alternative splicing. | ||
Elife Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer. | ||
Cell Signal Silencing ESRP1 expression promotes caspase-independent cell death via nuclear translocation of AIF in colon cancer cells.
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