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验证数据展示

  • IF Staining of HeLa using CL594-66192

    IF Staining of HeLa using CL594-66192

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 ERK1/2 antibody (CL594-66192, Clone: 4G8A11 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-66192

    FC experiment of HeLa using CL594-66192

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ERK1/2 (CL594-66192, Clone:4G8A11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated ERK1/2 Monoclonal antibody

ERK1/2 Monoclonal Antibody for IF/ICC
Cat No. CL594-66192

产品说明书

CloneNo. 4G8A11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC

别名

ERK1, ERK, ERK2, ERK-1, EC:2.7.11.24

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66192-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66192 targets ERK1/2 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 ERK1/2 fusion protein Ag20350 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 3
别名 ERK1, ERK, ERK2, ERK-1, EC:2.7.11.24
计算分子量 43 kDa
GenBank蛋白编号BC013992
基因名称 ERK1
Gene ID (NCBI) 5595
RRIDAB_2883530
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP27361
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

实验方案

Product Specific Protocols
IF protocol for CL594 ERK1/2 antibody CL594-66192Download protocol
Standard Protocols
Click here to view our Standard Protocols