验证数据展示
经过测试的应用
| Positive WB detected in | PC-12 cells, HeLa cells, Jurkat cells, RAW 264.7 cells, ROS1728 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 77 publications below |
| IHC | See 1 publications below |
产品信息
67170-1-Ig targets ERK1/2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human, mouse, rat, rabbit, chicken |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase 3 |
| 别名 | ERK, ERK 1, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3 |
| 计算分子量 | 38-43 kDa |
| GenBank蛋白编号 | NM_002746 |
| 基因名称 | ERK1 |
| Gene ID (NCBI) | 5595 |
| RRID | AB_2882466 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P27361 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
发表文章
| Species | Application | Title |
|---|---|---|
J Hepatol Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD | ||
Nat Cell Biol Defective prelamin A processing promotes unconventional necroptosis driven by nuclear RIPK1 | ||
Nat Commun SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation | ||
Nat Commun A glucose kinase-independent HK2 activity prevents TNF-induced cell death by phosphorylating RIPK1. | ||
Mol Cell Palmitoylation licenses RIPK1 kinase activity and cytotoxicity in the TNF pathway |
